Journal: RSC Advances

Article Title: Novel diazenyl chalcones with a 2-arylidene hydrazineylidene thiazole moiety: synthesis, anticancer potential, spectroscopic characterisation, and molecular docking studies

doi: 10.1039/d5ra07769a

Figure Lengend Snippet: Fluorescent microscopy analysis of HepG2 cells treated with synthetic compounds (4–6a,b) and stained with acridine orange/ethidium bromide (AO/EB).

Article Snippet: Normal human skin fibroblasts (HSF), the human breast cancer epithelial cell line (MDA), and the human liver cancer cell line (HepG2) were purchased from VACCERA Egypt and obtained from the American Type Culture Collection (ATCC).

Techniques: Microscopy, Staining

Journal: RSC Advances

Article Title: Novel diazenyl chalcones with a 2-arylidene hydrazineylidene thiazole moiety: synthesis, anticancer potential, spectroscopic characterisation, and molecular docking studies

doi: 10.1039/d5ra07769a

Figure Lengend Snippet: (A) Microscopic view of the cell migration inhibition assay of the HepG2 cell line after 48 hours. (B) Quantitative analysis of the wound area by calculating the wound closure percentage and migration rate after 48 hours, compared to the initial time (0 hours). (C) Relative fold change in the gene expression of MMP-2 and MMP-9 in cells treated with synthetic compounds (4–6a,b) in HepG-2. All assays were conducted in triplicate, and the results are expressed as mean ± SEM. MMP: matrix metalloproteinase.

Article Snippet: Normal human skin fibroblasts (HSF), the human breast cancer epithelial cell line (MDA), and the human liver cancer cell line (HepG2) were purchased from VACCERA Egypt and obtained from the American Type Culture Collection (ATCC).

Techniques: Migration, Inhibition, Gene Expression

Journal: RSC Advances

Article Title: Novel diazenyl chalcones with a 2-arylidene hydrazineylidene thiazole moiety: synthesis, anticancer potential, spectroscopic characterisation, and molecular docking studies

doi: 10.1039/d5ra07769a

Figure Lengend Snippet: Structure–activity relationship (SAR) of the synthesised azo derivatives (4–6a,b) showing the effect of aromatic substitution on cytotoxic selectivity. Selectivity index (SI) values against MDA-MB-231 and HepG2 cells relative to WI-38 normal cells are shown.

Article Snippet: Normal human skin fibroblasts (HSF), the human breast cancer epithelial cell line (MDA), and the human liver cancer cell line (HepG2) were purchased from VACCERA Egypt and obtained from the American Type Culture Collection (ATCC).

Techniques: Activity Assay